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Pelicluster CD66acde, 1ml

Monoclonal/ Polyclonal: Monoclonal
Clone: CLB-gran/10, IH4Fc
Host: Mouse
Isotype: IgG1
Application: Flow cytometry (FC)
Application notes: Study of mature granulocytes in peripheral blood. Methods: Indirect immunofluorescence staining with analysis by flow cytometry or fluorescence microscopy.
Conjugation Type: Conjugated
Conjugate: FITC
Conjugation note: FITC conjugated Goat anti-Mouse immunoglobulin antiserum, solid phase adsorbed with human immunoglobulins or comparable product. Remove aggregates by centrifugation at 1000 x g for 10 minutes. Dilute as mentioned in the procedure.
Purified: YES
Purification note: Culture supernatant purified by affinity chromatography
Buffer: Wash- and dilution buffer for mononuclear cells,(PBS/BSA): Phosphate Buffered Saline containing 0.2% BSA (w/v). Wash- and dilution buffer for platelets, (Seq).  Sequestrine buffer, storage 1 month at 2-8°C.  10 x stock solution, dissolve in 5
Gene synonyms: CD66c
UNSPSC code: 41110000

Pelicluster CD66acde, 1ml

Pelicluster CD66acde, 1ml

This clone has been derived from hybridisation of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with human granulocytes. The antibody was submitted to CD66acde in the Fifth and Sixth International Workshop on Human Leukocyte Differentiation Antigens. Source: Culture supernatant purified by affinity chromatography.  Molecular mass: 180-200 kDa (phosphoprotein), 90 kDa (PI-linked protein).The monoclonal mouse antibody is directed against the CD66acde antigen, which is expressed on mature human granulocytes. After granulocyte activation the expression is strongly increased. The monoclonal moue antibody reacts with 100% of mature human peripheral granulocytes. It reacts weak with malignant cells of patients with B cell derived Chronic Lymphoid Leukaemia (CLL).  The monoclonal mouse antibody does not react with normal human peripheral  B cells, T cells, monocytes and platelets. In immunohistology the monoclonal mouse antibody reacts with some tissue macrophages and CEA, the Carcino Embryonic Antigen, expressed on colon carcinoma and other carcinomas (1-3).