PeliClass human IgG Subclass Plus kit

Kit for quantitative determination of human IgG subclasses in serum and plasma on the Beckman IMMAGE / IMMAGE 800.

Article number	M1895
Product group	IgG subclasses IMMAGE
Technique	Nephelometry/turbidimetry

Buy now

Product information

General information

Human IgG contains of four subclasses: IgG1, IgG2, IgG3 and IgG4. Different types of IgG antibodies, called subclasses, have been studied extensively in terms of their biochemical characteristics^1-5. These subclasses have important functions in the body, such as recognizing antigens (foreign substances), activating the complement system (part of the immune response), and binding to receptors on cell surfaces. Research has shown that abnormalities in the levels of these IgG subclasses in the blood may be related to various diseases⁶.

For example, a deficiency in the IgG2 subclass has been linked to an increased susceptibility to viral or bacterial infections. People with low levels of IgG2 or IgG3 in their blood have been found to have recurrent upper and lower respiratory tract infections^4,5. Similarly, very low levels of IgG4 have been associated with recurring infections in the sinuses and lungs⁷.

Abnormalities in the levels of IgG subclasses have also been observed in autoimmune diseases, neurological disorders, and HIV infections^4,6. These findings suggest that monitoring the levels of these subclasses can provide valuable information about a person’s immune health and help diagnose or understand certain diseases.

Buy now

Test principles

The PeliClass human IgG subclass Plus kit is designed to quickly and accurately measure different types of human IgG antibodies (IgG1, IgG2, IgG3, and IgG4). The kit uses two different methods: nephelometric determination for IgG1 and IgG2, and turbidimetric determination for IgG3 and IgG4.

In the nephelometric method, a special antiserum that specifically reacts with IgG1 and IgG2 is used. This creates a reaction that can be measured by the amount of scattered light. In the turbidimetric method, a latex reagent coated with specific antibodies for IgG3 and IgG4 is used. This creates a reaction that can be measured by the amount of light passing through.

To ensure accuracy, the antibodies used in the kit are carefully selected for their high avidity (strong binding). They are made specific to human IgG subclasses by removing unwanted antibodies through absorption with isolated gammaglobulins and serum fractions.

To determine the IgG subclass concentrations in test samples, a reference curve is used. This curve is created by comparing the measurements of provided IgG subclass calibrators with known concentrations. Control sera are also included in the kit to verify the accuracy of the measurements.

The assigned values for the IgG subclass concentrations in the calibrators are provided by the World Health Organization (WHO) and are derived from a specific reference (WHO 67/97).

Overall, the PeliClass human IgG subclass Plus kit offers a reliable and standardized way to measure IgG subclasses, providing valuable information about the immune response.

Storage and stability

All the components of the kit remain stable until the expiration date indicated on the label when stored at 28°C. It’s important to note that transportation conditions may be different from storage conditions. The IgG3 and IgG4 latex reagents should not be frozen.

Once opened, the components can be stored at 2-8°C for one month, considering the expiration date on the label. After completing the daily workload, make sure to return the components to 28°C. Before storing the reagent cartridges, replace the evaporation caps with screw caps to prevent evaporation.

The calibration curves remain stable for at least one month, but it’s always recommended to use control sera to verify the accuracy of the calibration curves.

Package contents

The PeliClass human IgG subclass Plus kit enables you to measure the amounts of four different types of human IgG antibodies in 50 tests. This includes calibrators and controls. If needed, you can order each component separately as mentioned on the first page of the package insert.

The IgG1 and IgG2 antisera are special liquid sheep sera that specifically react with IgG1 and IgG2 antibodies. The IgG3 and IgG4 latex reagents are suspensions of tiny polystyrene particles coated with specific sheep antibodies that target IgG3 and IgG4 antibodies. The calibrators and controls are liquid human sera.

All components in the kit contain a preservative called NaN3 at a concentration of 0.1% (w/v).

Buy now

Sheep antibodies against human IgG1	1x 2.4 mL	M1891
Sheep antibodies against human IgG2	1x 2.9 mL	M1892
Latex enhanced sheep antibodies against human IgG3	1x 4.0 mL	M1893
Latex enhanced sheep antibodies against human IgG4	1x 4.0 mL	M1894
Human IgG subclass calibrator set	7x 1.0 mL	M1896
Human IgG subclass control 1	1x 1.0 mL	M1897
Human IgG subclass control 2	1x 1.0 mL	M1898

Precautions

The calibrators and controls in this kit are made from liquid human sera. These human sera have been tested for specific disease-causing agents according to EU guidelines and Good Manufacturing Practices (GMP). The tests confirm that they do not show any signs of infection. However, it’s important to note that since these components come from human sources, they should be considered potentially infectious. Proper waste disposal should be carried out in accordance with your laboratory regulations.

Please be aware that the reagent in this kit cannot be guaranteed to be free from infectious agents. Therefore, precautions should be taken when handling it.

All the components of the kit remain stable until the expiration date mentioned on the label when stored at 2-8°C. Keep in mind that transportation conditions may differ from storage conditions, so take appropriate measures to maintain the required temperature range. It is also important not to freeze the IgG3 and IgG4 latex reagents.

Buy now

Test sample handling

You can test serum and plasma samples (EDTA and Na-heparin) using this kit. It is recommended to use fresh samples, but if that’s not possible, they can be stored at 2-8°C. If the samples won’t be tested within 1 week, they should be frozen and kept at a temperature between -18°C and -30°C. They can be stored in the freezer for up to 3 months. It’s important to avoid subjecting the samples to repeated freeze-thaw cycles, as it can affect the accuracy of the results.

If the samples appear lipaemic or turbid, they need to be clarified before testing. This can be done by centrifugation. If the samples cannot be clarified, they should not be used for testing. Additionally, it is generally not necessary to manually dilute the samples before using them, unless otherwise instructed.

Buy now