PeliClass latex sheep anti human IgG3

Kit for quantitative determination of human IgG subclasses in serum and plasma on the Beckman IMMAGE / IMMAGE 800.

Article number M1893
Product group IgG subclasses IMMAGE
Technique Nephelometry/turbidimetry

General information

The four subclasses of Human IgG are IgG1, IgG2, IgG3, and IgG4. Comprehensive descriptions of the biochemical characteristics of these IgG subclasses are available in the literature 1-5. These subclasses exhibit variations that have significant implications for various essential biological functions such as antigen recognition, complement activation, and binding to cell surface receptors. Numerous studies have indicated potential links between abnormalities in serum levels of IgG subclasses and various disease conditions 6.

Notably, there is substantial documented evidence highlighting the connection between selective IgG2 subclass deficiency and an elevated susceptibility to viral or bacterial infections 4,5. Patients with recurrent upper and lower respiratory tract infections have been found to exhibit low serum levels of IgG2 or IgG3. Additionally, research has pointed to a correlation between notably low IgG4 serum concentrations and recurring sino-pulmonary infections 7.

Furthermore, disturbances in serum levels of IgG subclasses have been observed in autoimmune disorders, neurological conditions, and HIV infections 4,6. These findings underscore the critical role of IgG subclasses in immune responses and their potential as indicators for various health states.

Test principles

Presenting the PeliClass Human IgG Subclass Plus Kit, expertly designed for swift and accurate quantification of IgG subclasses – IgG1, IgG2, IgG3, and IgG4.

IgG1 and IgG2 levels are measured nephelometrically using a monospecific anti-IgG subclass antiserum. IgG3 and IgG4 quantification relies on a turbidimetric approach with latex reagents coated in highly avid anti-IgG subclass antibodies, enhancing sensitivity.

Our kit ensures specificity through refined fractionated sheep anti-human IgG subclass sera, absorbed to eliminate unwanted subclasses. Polyclonal reagents, chosen for their avidity, power the nephelometric and turbidimetric measurements based on immune complex formation.

Accurate IgG subclass concentrations are determined by comparing test samples to provided calibrators. Quality control is maintained through IgG subclass control sera. Calibration is based on ERM-DA470, yielding IgG1 (6.210 g/L), IgG2 (3.450 g/L), IgG3 (0.390 g/L), and IgG4 (0.591 g/L) values from WHO 67/97 11.

Discover precise immune response insights with the PeliClass Human IgG Subclass Plus Kit.

Storage and stability

Components remain stable until the expiration date indicated on the label when stored between 2-8°C. Please note that transportation conditions may vary from storage conditions. Avoid freezing the IgG3 and IgG4 latex reagents. Upon opening and storage at 2-8°C, components retain stability for a duration of 1 month, considering the expiration date on the label. Following the daily workload, ensure components are returned to the 2-8°C storage.

When storing reagent cartridges, replace evaporation caps with screw caps to maintain integrity. Calibration curves maintain stability for at least 1 month. However, always utilize control sera to verify the calibration curve’s accuracy.

Package contents

The PeliClass human IgG subclass Plus kit enables you to measure the amounts of four different types of human IgG antibodies in 50 tests. This includes calibrators and controls. If needed, you can order each component separately as mentioned on the first page of the package insert.

The IgG1 and IgG2 antisera are special liquid sheep sera that specifically react with IgG1 and IgG2 antibodies. The IgG3 and IgG4 latex reagents are suspensions of tiny polystyrene particles coated with specific sheep antibodies that target IgG3 and IgG4 antibodies. The calibrators and controls are liquid human sera.

All components in the kit contain a preservative called NaN3 at a concentration of 0.1% (w/v).

Sheep antibodies against human IgG1

1x 2.4 mL


Sheep antibodies against human IgG2

1x 2.9 mL


Latex enhanced sheep antibodies against human IgG3

1x 4.0 mL


Latex enhanced sheep antibodies against human IgG4

1x 4.0 mL


Human IgG subclass calibrator set

7x 1.0 mL


Human IgG subclass control 1

1x 1.0 mL


Human IgG subclass control 2

1x 1.0 mL M1898


The calibrators and controls consist of liquid human sera. While these sera have been screened for disease-transmitting markers in compliance with prevailing EU GMP guidelines and determined nonreactive, it’s important to regard all human-origin components as potentially infectious. Proper waste disposal must adhere to your laboratory’s regulations.

Please note that the reagent cannot be guaranteed free from infectious agents.

Stability of all components extends until the expiration date indicated on the label when stored within 2-8°C. Be aware that transport conditions may differ from storage requirements. Ensure not to freeze the IgG3 and IgG4 latex reagents.

Test sample handling

Both serum and plasma (EDTA and Na-heparin) are suitable for testing. It’s advisable for samples to be as freshly obtained as possible or stored at temperatures between 2-8°C. In cases where testing is delayed beyond 1 week, freezing at temperatures ranging from -18°C to -30°C is recommended. Samples can remain stored in this manner for a maximum of 3 months. However, bear in mind that repeated freeze-thaw cycles can potentially compromise the analyte’s quality.

For lipemic or turbid samples, centrifugation is necessary to achieve clarity before testing. If samples cannot be clarified, they should be excluded from use. It’s important to avoid manual dilution of samples before use unless explicitly instructed otherwise.


Find out more information about the scientific background of the product.

Shakib F. (1986)

(Volume editor) Basic and Clinical Aspects of IgG Subclasses (Monographs in Allergy 19) Karger.

View study

Shakib F. (1990)

(Editor) The human IgG subclasses Pergamon Press.

View study

Vlug A. et al. (1989)

Eur. Clin. lab. 8: 26.

Jefferis R. and Kumararatne D.S. (1990)

Clin. Exp. Immunol. 81: 357.

Hamilton R.C. (1987)

Clin. Chem. 33: 1707.

Vries E. de et al. (2006)

Clin. Exp. Immunol. 145: 204.

Beck C.S. and Heiner D.C. (1981)

Am. Rev. Respir. Dis. 124: 94.

Giessen M. et al. (1974)

Immunology 27: 655.

Goossen P.C.M. et al. (1981)

J. Immunol. Methods 40: 339.

Vlug A. et al. (1994)

Ann. Biol. Clin. 52: 561.

Klein F. et al. (1985)

Clin. Chem. Acta. 150: 119.

Lepage N. et al. (2010)

Clin. Biochem. 43: 694.

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