PeliClass latex sheep anti human IgG4

Kit for quantitative determination of human IgG subclasses in serum and plasma on the Beckman IMMAGE / IMMAGE 800.

Article number M1894
Product group IgG subclasses IMMAGE
Technique Nephelometry/turbidimetry

General information

Human IgG consists of four subclasses: IgG1, IgG2, IgG3, and IgG4. Extensive research has been conducted to describe the biochemical characteristics of these IgG subclasses 1-5. These subclasses exhibit differences that are crucial for various biologically significant functions, including antigen recognition, complement activation, and binding to cell surface receptors. Numerous studies have indicated a potential link between abnormal serum levels of IgG subclasses and various disease conditions 6.

One particularly notable association is the connection between selective IgG2 subclass deficiency and an increased susceptibility to viral or bacterial infections, a topic that has been thoroughly documented 4,5. Patients with recurrent upper and lower respiratory tract infections have been found to have low serum levels of IgG2 or IgG3. Additionally, some studies have reported a correlation between very low IgG4 serum concentrations and recurrent sino-pulmonary infections 7. Moreover, abnormalities in the serum levels of IgG subclasses have been observed in autoimmune diseases, neurological disorders, and HIV infections 4,6.

Test principles

Introducing the PeliClass Human IgG Subclass Plus Kit, a cutting-edge solution designed to facilitate rapid, consistent, and precise quantification of human IgG subclasses, including IgG1, IgG2, IgG3, and IgG4.

The quantification of IgG1 and IgG2 in serum is accomplished through nephelometric determination, relying on the specific reaction with a monospecific, highly avid anti-IgG subclass antiserum. For enhanced analytical sensitivity, the turbidimetric determination of IgG3 and IgG4 in serum utilizes a latex reagent coated with monospecific, highly avid anti-IgG subclass antibodies.

To ensure specificity, the fractionated sheep anti-human IgG subclass sera are meticulously prepared by absorption with isolated gammaglobulins of unwanted IgG subclasses and, if necessary, through absorption with isolated serum fractions 8-10. The polyclonal reagents incorporated in this kit are meticulously selected for their high avidity and specificity for human IgG subclasses. The nephelometric and turbidimetric quantifications operate on the principle of immune complex generation, measured by scattered light and light passing through, respectively.

Accurate determination of IgG subclass concentrations in test samples is achieved by comparing the results with a reference curve, generated using the provided IgG subclass calibrators. Ensuring the accuracy of the IgG subclass determinations and the validity of the reference curves, IgG subclass control sera are assayed.

For the calibration of IgG subclass levels in the calibrators, the ERM-DA470 serves as the calibrator, with assigned values of 6.210 g/L for IgG1, 3.450 g/L for IgG2, 0.390 g/L for IgG3, and 0.591 g/L for IgG4, derived from WHO 67/97 11.

With the PeliClass Human IgG Subclass Plus Kit, researchers and professionals can confidently analyze human IgG subclasses with efficiency and accuracy, backed by references 8-11 attesting to its reliable performance.

Storage and stability

Store all components at 2­-8°C until the labeled expiry date. Do not freeze IgG3 and IgG4 latex reagents. After opening, they remain stable at 2-­8°C for 1 month. Return components to 2-­8°C after daily use.

Replace evaporation caps with screw caps before storing reagent cartridges. Calibration curves are stable for at least 1 month, but validate with control sera. Contact us for any questions.

Package contents

Introducing the PeliClass Human IgG Subclass Plus Kit – a powerful tool for quantitatively determining the four human IgG subclasses in 50 tests, complete with calibrators and controls.

Additionally, all components listed in the package insert’s first page are available for individual purchase.

Sheep antibodies against human IgG1

1x 2.4 mL


Sheep antibodies against human IgG2

1x 2.9 mL


Latex enhanced sheep antibodies against human IgG3

1x 4.0 mL


Latex enhanced sheep antibodies against human IgG4

1x 4.0 mL


Human IgG subclass calibrator set

7x 1.0 mL


Human IgG subclass control 1

1x 1.0 mL


Human IgG subclass control 2

1x 1.0 mL M1898


The calibrators and controls consist of liquid human sera. While these human sera have undergone testing for specific disease transmitting agents following EU guidelines to GMP and have been found nonreactive, it is essential to treat all human-origin components as potentially infectious. Proper waste disposal should be carried out in accordance with your laboratory regulations.

Please note that the reagent cannot be guaranteed to be free from infectious agents.

For optimal stability, store all components at 2­-8°C until the expiry date mentioned on the label. Please be aware that transport conditions may differ from storage conditions. To preserve the integrity of the IgG3 and IgG4 latex reagents, avoid freezing them.

Test sample handling

Our test can be conducted on both serum and plasma samples (EDTA and Na-heparin). For optimal results, it is recommended to use fresh samples or store them at 2­-8°C until testing. If the samples are not tested within 1 week, they should be frozen and stored at ­18°C to ­30°C, with a storage duration of up to 3 months. However, keep in mind that subjecting the samples to repeated freeze-thaw cycles may compromise the analyte’s integrity.

To ensure accurate testing, lipaemic or turbid samples must be clarified through centrifugation before analysis. If a sample cannot be clarified, it should not be used for testing. Manual dilution of the samples should generally be avoided unless specifically instructed otherwise.


Find out more information about the scientific background of the product.

Shakib F. (1986)

(Volume editor) Basic and Clinical Aspects of IgG Subclasses (Monographs in Allergy 19) Karger.

View study

Shakib F. (1990)

(Editor) The human IgG subclasses Pergamon Press.

View study

Vlug A. et al. (1989)

Eur. Clin. lab. 8: 26.

Jefferis R. and Kumararatne D.S. (1990)

Clin. Exp. Immunol. 81: 357.

Hamilton R.C. (1987)

Clin. Chem. 33: 1707.

Vries E. de et al. (2006)

Clin. Exp. Immunol. 145: 204.

Beck C.S. and Heiner D.C. (1981)

Am. Rev. Respir. Dis. 124: 94.

Giessen M. et al. (1974)

Immunology 27: 655.

Goossen P.C.M. et al. (1981)

J. Immunol. Methods 40: 339.

Vlug A. et al. (1994)

Ann. Biol. Clin. 52: 561.

Klein F. et al. (1985)

Clin. Chem. Acta. 150: 119.

Lepage N. et al. (2010)

Clin. Biochem. 43: 694.

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