PeliCluster Anti-Granzyme B

Description:

Anti-Granzyme B (clone GB11) is a mouse monoclonal antibody targeting the serine protease Granzyme B. This enzyme is abundantly expressed in cytotoxic T lymphocytes and NK cells, where it plays a central role in apoptosis and immune defense.

Target:

Granzyme B is a 32 kDa serine protease stored in cytotoxic granules. Expression is mainly cytoplasmic in activated T cells and NK cells, with low levels reported in granulocytes, B cells, and dendritic cells.

The Anti-Granzyme B antibody (clone GB11) has been validated in flow cytometry. Additional applications such as ELISA or immunohistochemistry may be feasible but require user validation. It is recommended to titrate the antibody in each chosen assay using appropriate controls.

For further information or technical support, please contact us.

For research use only!

Product: Anti-Granzyme B
Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0
Concentration: 2 mg/mL
Preservative: None

Find out more information about the scientific background of the product.

• Mattila JT, Maiello P, Sun T, Via LE, Flynn JL. Granzyme B-expressing neutrophils correlate with bacterial load in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques. Cell Microbiol 2015; 17: 1085–1097. PMID:25653138.

• Wever PC, Van Der Vliett HJJ, Spaeny LHA, Wolbink AM, Van $ F N J, Froelich DCJ et al. The CD8 + granzyme B + T-cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells. 1998.

• Thiery J, Keefe D, Saffarian S, Martinvalet D, Walch M, Boucrot E et al. Perforin activates clathrin-and dynamin-dependent endocytosis, which is required for plasma membrane repair and delivery of granzyme B for granzyme-mediated apoptosis. 2010.

• Kummer JA, Kamp AM, Van Katwijk M, Brakenhoff JPJ, Rado~evi6 K, Marie Van Leeuwen A et al. Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins *. 1993.

• Berjis A, Muthumani D, Aguilar OA, Pomp O, Johnson O, Finck A V. et al. Pretreatment with IL-15 and IL-18 rescues natural killer cells from granzyme B-mediated apoptosis after cryopreservation. Nat Commun 2024; 15. PMID:38729924.

• Ahern DJ, Ai Z, Ainsworth M, Allan C, Allcock A, Angus B et al. A blood atlas of COVID-19 defines hallmarks of disease severity and specificity. Cell 2022; 185: 916-938.e58. PMID:35216673.

• Hoang TN, Pino M, Boddapati AK, Viox EG, Starke CE, Upadhyay AA et al. Baricitinib treatment resolves lower-airway macrophage inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques. Cell 2021; 184: 460-475.e21. PMID:33278358.

• Van Dongen JJM, Lhermitte L, Böttcher S, Almeida J, Van Der Velden VHJ, Flores-Montero J et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 2012; 26: 1908–1975. PMID:22552007.

• Lin L, Couturier J, Yu X, Medina MA, Kozinetz CA, Lewis DE. Granzyme B secretion by human memory CD4 T cells is less strictly regulated compared to memory CD8 T cells. BMC Immunol 2014; 15. PMID:25245659.

• Jahrsdö B, Vollmer A, Blackwell SE, Maier J, Sontheimer K, Beyer T et al. Granzyme B produced by human plasmacytoid dendritic cells suppresses T-cell expansion. 2010; 115: 1156–1165.

• Rong J, Xu X, Ewen C, Chris Bleackley R, Kane KP. Isolation and Characterization of Novel Single-Chain Fv Specific for Human Granzyme B. 2004.

• Spaeny-Dekking EHA, Hanna WL, Wolbink AM, Wever PC, Kummer AJ, Swaak AJG et al. Extracellular Granzymes A and B in Humans: Detection of Native Species During CTL Responses In Vitro and In Vivo. The Journal of Immunology 1998; 160: 3610–3616. PMID:9531325.