Precision vWF Immunoprecipitation & Immunoblotting ELISA Kit, accurate hemostasis analysis and von Willebrand disease research.

Streptavidin poly‐HRP, 1mL

Streptavidin conjugated to Poly-Horseradish Peroxidase.

Article number M2032
Product group Immune reagents
Size 1 mL

Characteristics

Streptavidin conjugated to Poly-Horseradish Peroxidase. Composition: Streptomyces avidinii. The HRP-conjugated streptavidin binds to biotin.

Conjugate concentration: 1.0 mg/mL
Molecular E/P ratio: 20.0
The HRP-conjugated streptavidin was found specific for biotin in ELISA and ELISPOT techniques.

Precautions

Leaking or damaged vials must not be used. The reagent cannot be assumed to be free from infectious agents. Care must be taken in the use and disposal of each container and its contents. Waste-disposal, after completion of the test, should be performed according to your laboratory regulations. Poly-HRP-conjugated streptavidin should be stored below –18°C. Repeated thawing and freezing may reduce the antibody activity and should be avoided. The reagent is stable until the expiry date stated on the vial label. Spin small volumes before use.

Package contents

M2032: Each vial contains 1.0 mL Poly-HRP-conjugated streptavidin. Preservative: 5-Nitro-5-Bromo-1,3Dioxan (600 ppm).

Application

Detection of biotinylated reagents in ELISA, ELISPOT and immunostaining techniques.

1. Store the stock solution below –18°C.

2. Before use: dilute the required quantity of the poly-HRP with dilution buffer and immediately restore the stock solution below –18°C.

3. A concentration of 1% casein in the dilution buffer is recommended to exclude an increased background.

4. For optimal results with ELISA techniques HPE buffer (M1940) is recommended.

Streptavidin poly HRP refers to a conjugate of streptavidin with polymerized horseradish peroxidase (HRP), designed to enhance the detection sensitivity in various bioanalytical assays, such as enzyme-linked immunosorbent assays (ELISAs), Western blots, and nucleic acid hybridizations. Streptavidin is highly valued for its strong affinity to biotin, making it an essential tool for binding biotinylated molecules. When coupled with the enzymatic activity of HRP, this conjugate facilitates amplified signal generation, leading to improved visualization and quantification of target molecules. This combination is particularly useful in applications requiring high sensitivity and specificity.

Storage

hemostasis analysis

Store the stock solution below –18°C

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